Lightsheet Microscopy

What is LSFM?

Light sheet fluorescence microscopy (LSFM) illuminates biological specimens with a plane of light for high-throughput serial acquisition of 3D images.

Structured Illumination (SI)

We use a 1D spatial light modulator called a grating light valve to produce illumination patterns used in optical sectioning structured illumination microscopy.

Sample Interface

An open-top biological interface is enabled using a hypohemispherical immersion lens to limit aberrations when obliquely imaging across a flat glass window.

Postprocessing

In order to perform postprocessing, we are building realtime GPU routines to quickly deskew and deconvolve the raw image data.

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